Curriculum L. Astrologo

Letizia Astrologo

January 30, 1987, Rome, Italy
Italian citizen

Work address: Sapienza University of Rome, Department of Biology and Biotechnologies "Charles Darwin", P.le A. Moro 5, 00185 Rome, Italy & Department of Molecular Medicine, V.le Regina Elena 324, 00161 Rome, Italy
Phone number: +39 0649912849/472
Fax: +39 064456866
E-mail: letizia.astrologo@uniroma1.it; astrolety@libero.it
Skype contact: letizia.astrologo

Education
November 2010-today: PhD studentship in Physiopathology of Skeletal Tissues, Sapienza University of Rome. Supervisors: Prof. Paolo Bianco and Prof. Isabella Saggio

October 2010: Laurea cum laude in Genetics and Molecular Biology, Sapienza University of Rome. Degree Thesis title: "Analysis of transcriptional modulation induced by human and canine adenoviral vectors in human neuronal cells". Supervisor: Prof. Isabella Saggio

December 2008: Laurea cum laude in Biological Sciences, Sapienza University of Rome. Degree Thesis title: "Study of an in vitro model of genetic disease Fibrous Dysplasia". Supervisor: Prof. Isabella Saggio

July 2005: Scientific High School Degree (Score 100/100), Liceo Renzo Levi, Rome, Italy

Professional experiences
2010-today: PhD student, research activity, Sapienza University of Rome. Training coordinators: Prof. Isabella Saggio and Prof. Paolo Bianco. Research work: study of in vitro and in vivo models of Fibrous Dysplasia disease

2008-2010: Research activity, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome. Training coordinator: Prof. Isabella Saggio. Research work: high-throughput transcriptional analysis of gene therapy viral vectors effects on brain cells (http://www.braincav.eu)

2008: Student training, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome. Training coordinator: Prof. Isabella Saggio. Research work: transfer of the Fibrous Dysplasia genotype in human skeletal progenitors and in vitro analysis

2006/2007: Scholarship at the structure of didactic labs of Department of Animal and Human Biology (BAU) of Sapienza University of Rome.

Posters and presentations
Piersanti S., Astrologo L., Schwarz S.C., Schwarz J., Chillon M., Kremer E.J., Saggio I. High-throughput transcriptional analysis of gene therapy viral vectors effects on brain cells. Poster, 18th European Society of Gene and Cell Therapy Annual Congress 2010. Milan, Italy (October 2010)

Laboratory skills
Cell Cultures
Cell Transfection
Lentiviral Vector Production and Titration
Lentivirus-mediated transduction
Adenoviral Vector Production and Titration
Adenovirus-mediated transduction
PCR, RT-PCR, Real Time PCR
Genomic DNA isolation from cells and tissues
RNA extraction from cells and tissues and retrotranscription
Protein extraction from cell
Western Blotting
Bacterial transformation
Plasmid DNA Purification (Maxiprep, Midiprep)
ELISA assay

Computer Knowledge (Windows, Mac)
Microsoft Office package
Adobe Photoshop CS3
Browser web: Microsoft Internet Explorer, Firefox, Safari, Google Chrome
DNAman
Quantity one
MetaMorph
Endnote 9
GenoPro
Microarray analysis: Expression Analysis Sistematic Explorer (David), Ingenuity Pathway Analysis

Foreign Languages
English, Hebrew