Curriculum V. Cacciafesta

Name: Valeria Cacciafesta

February 8, 1989, Rome, Italy

Italian citizen

Work address: Department of Biology and Biotechnologies "Charles Darwin" Sapienza

University of Rome,

P.le A. Moro 5, Rome, Italy

Phone number: +39 0649912849/472

Fax: +39 064456866

E-mail: [email protected]

Education:

October 2011: Laurea cum laude in Biological Sciences, Sapienza University of Rome. Degree Thesis title: "L’utilizzo di cellule staminali nella cura delle malattie neurodegenerative". Supervisor: Prof. Laura Conti

July 2007: High School Degree (Score 98/100), Liceo Ginnasio Giulio Cesare, Rome, Italy

Professional experiences:

2012- present day: Student training, Department of Biology and Biotechnologies "Charles

Darwin", Sapienza University of Rome. Training coordinator Prof. Isabella Saggio.

Laboratory skills
Cell cultures (cell lines)
Cell transfection
Lentiviral vector production and titration
Genomic DNA isolation from cells and tissues
RNA extraction from cells and tissues and retrotranscription
PCR, RT-PCR

Protein extraction from cells
Western Blotting
Bacterial transformation
Plasmid DNA purification (Midiprep)
ELISA assay
Mouse management and manipulation

Computer Knowledge (Windows, Mac)
Browser web: Microsoft Internet Explorer, Firefox, Google Chrome
Microsoft Office package
Adobe Photoshop CS5.1
ApE – A plasmid Editor
Quantity one
GenoPro

Foreign language:

- English