Giovanna Di Ruocco
Jan. 2020 – to date Master student in Stem Cells and Genome Editing, Sapienza University of Rome, Italy
2017 Master’s degree in Genetics and Molecular Biology for Basic and Biomedical Research, Sapienza University of Rome, Italy
2013 Bachelor’s degree in Biological Sciences Sapienza University of Rome, Italy
Sept. 2020 – to date Master internship in biomedical research, Immune response in the elderly to a COVID19 vaccine candidate. Supervisor: Prof. Isabella Saggio, Tutor: Dr. Mattia La Torre, Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, Italy.
Jan. 2015 – Oct. 2017 Undergraduate research experience in basic research, comparative genomics of plant model systems.
Supervisor: Dr. Raffaele Dello Ioio, Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, Italy.
During this experience I had the chance to learn and work in other two renowned laboratories different from mine:
22 Aug. – 3 Sept. 2016 In Situ Hybridization experiments on whole mount and tissue sections of Cardamine hirsuta.
Supervisor: Dr. Ikram Blilou, Plant Developmental Biology, Wageningen University and Research, The Netherlands.
Jun. – Jul. 2017 Adaptation of experimental protocols for mRNA In Situ Hybridization assays and experiments on tissue sections of Cardamine hirsuta roots.
Supervisor: Prof. Maura Cardarelli, Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, Italy.
· Molecular methods and techniques: media preparation, agarose gel electrophoresis, DNA and RNA extraction, genotyping, PCR, synthesis of riboprobe, Cell culture: E. Coli and A. Tumefaciens, DNA cloning strategies: Classic and Gateway cloning systems, Generation of transgenic plants.
· Plant Farming, Plant Crossing techniques, Plant selection and Seeds collection in both species Arabidopsis thaliana and Cardamine hirsuta.
· Microscopy methods and techniques: DIC microscopy, confocal microscopy.
· Immunocytochemistry mehtods and techniques: mRNA In Situ Hybridization technique on whole mount and tissue sections of plant samples, Digoxigenin-Based immunoassays.
· MS Office (Word, Excel, PowerPoint) and Adobe Creative Suite 6 (Photoshop, Illustrator, Acrobat X).
· Online databases researches: The Arabidopsis Information Resource (TAIR), Arabidopsis eFP Browser, Salk Institute Genomic Analysis Institute (SiGnAL), The European Arabidopsis Stock Centre (uNASC), Protein Data Bank.
· Bioinformatics tools: Blast, Integrated Genome Browser, CLC Sequence viewer, ApE – A plasmid Editor v.2.0.47 and MAFFT Multiple Sequence Alignment.
Publications in journals
· Di Ruocco G, Di Mambro R, Dello Ioio R. Building the differences: a case for the ground tissue patterning in plants. Proc Biol Sci. 2018;285(1890):20181746. Published 2018 Nov 7. doi:10.1098/rspb.2018.1746
· Di Ruocco G, Bertolotti G, Pacifici E, et al. Differential spatial distribution of miR165/6 determines variability in plant root anatomy. Development. 2018;145(1):dev153858. Published 2018 Jan 9. doi:10.1242/dev.153858
Italian, English, French.