February 14, 1987, Anzio, Italy Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Italy klizia.maccaroni87@gmail.com |
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01/10/2019 – present
01/12/2018–31/09/2019
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Post-doctoral training Supervisor: Professor Isabella Saggio – Dept. of Biology and Biotechnologies “Charles Darwin” Sapienza University of Rome
Piazzale Aldo Moro 5, 00185 Rome (Italy)
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Molecular Cytogenetics Laboratory Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin” Sapienza University of Rome Piazzale Aldo Moro 5, 00185 Rome (Italy) |
01/11/2015–31/10/2018 |
Ph.D. in Genetics and Molecular Biology (XXXI cycle) – with fellowship |
Sapienza University of Rome Piazzale Aldo Moro 5, 00185 Rome (Italy) Thesis in molecular cytogenetics: “Common Fragile Sites: updating the causes of their variability in different cell tissues”. Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin” Tutor: Doctor Francesca Degrassi – IBPM – CNR – Rome Coordinator: Professor Fulvio Cruciani - Dept. of Biology and Biotechnologies “Charles Darwin” |
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EDUCATION AND TRAINING |
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11/2017–12/2017 |
Professional Practice Exam in Biology |
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Sapienza University of Rome Piazzale Aldo Moro 5, 00185 Rome (Italy) |
10/2013–03/2015 |
Master’s degree in Genetics and Molecular Biology in Basic and Biomedical Research |
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Sapienza University of Rome Piazzale Aldo Moro 5, 00185 Rome (Italy)
Master's degree research in molecular cytogenetic (110/110 cum laude): “Analysis of Common Fragile Sites expression in human lymphocytes and fibroblasts”. Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin” |
09/2007–07/2012 |
First degree in Biological Sciences |
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Sapienza University of Rome Piazzale Aldo Moro 5, 00185 Rome (Italy)
First degree dissertation in Cellular Biology: “Telomeres and Telomerase in cellular senescence and cancer”.
Supervisor: Professor Carla Cioni - Dept. of Biology and Biotechnologies “Charles Darwin” |
PERSONAL SKILLS |
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Native language |
Italian |
English |
Excellent written and oral production. Level B2 certified by First Certificate of English (FCE). |
Japanese |
Basic written and oral production. Level N5 certified by Japanese Language Proficiency Test (JLPT). |
Job-related skills |
▪ Cytogenetics: preparation of human karyotypes; recognition and analysis of human normal and pathological chromosomes; analysis of human chromosomal rearrangements and aberrations; Induction and classification of Common Fragile Sites (CFSs) in human cell lines; preparation of cell culture from lymphocytes from peripheral blood; preparation of normal and pathological human cell lines; preparation and analysis of metaphase spreads of human chromosomes; Fluorescence in situ hybridization (FISH) on human nuclei and metaphases; chromatin fibers preparation through fiber combing technique; R-banding on normal and pathological human karyotypes with Chromomycin A3; Giemsa staining on human chromosomes; G-banding staining on human chromosomes; immunostaining on human fixed chromosomes; preparation of DNA probes with Nick Translation; human nuclei staining with Ag-NOR; observation of metaphases spreads with optic microscopy and fluorescence microscopy with CCD camera; bacterial culture preparation. ▪ Molecular Biology: agarose and polyacrylamide gel electrophoresis; protein extraction and Western Blot; bacterial transformation; DNA extraction (BAC e PAC, plasmids); RNA extraction; real time- PCR; transfection on human cell lines. ▪ Informatics/Bioinformatics: Windows and Macintosh operative systems; Microsoft Office; Adobe Photoshop; Genomic and proteomic databases (NCBI, BLAST, PDB, GO, UniProt, RepeatMasker, OMIM, Mitelman); software and tools for modelling and prediction of proteins’ structure: ExPasy, Pymol 2.0 and PyMod. |
ADDITIONAL INFORMATION |
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Publications |
▪ Capitano F., Gargiuli C., Angerilli A., Maccaroni K., Pelliccia F., Mele A., Camilloni G., (2016). RNA polymerase I transcription is modulated by spatial learning in different brain regions. J. Neurochem. 136(4):706-716. ▪ Pelliccia F., Genovesi ML., Maccaroni K., (2015). Fanconi anaemia, chromosome instability, DNA replication and fragile sites. Eur. J Hum Genet, 23, suppl.1, 451. |
Poster |
▪ Joint meeting AGI-SIMAG – 26-28/09/2019. Common Fragile Sites: how the impaired replication timing promotes their tissue-specific expression. Maccaroni K., Balzano E., Mirimao F., Pelliccia F., (2019)
▪ CSHL (NY, USA) – 3-7/09 2019. Eukaryotic DNA Replication & Genome Maintenance. Maccaroni K., Balzano E., Mirimao F., Pelliccia F., (2019). Genome Instability at Common Fragile Sites. Updating the causes of their variability in different cell tissues.
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Conferences and congresses participation |
▪ Cortona (AR) – 26-28/09/2019 Joint Meeting AGI-SIMAG
▪ Cortona (AR) -14 -15/06/2018 Genetics School in Cortona: Cytogenetic and Molecular Citogenomics.
▪ Rome – 18-21/09/2018 XV FISV Congress (Federazione Italiana Scienze della Vita) |
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