February 14, 1987, Anzio, Italy

Department of Biology and Biotechnology “Charles Darwin”,

Sapienza University of Rome, Italy

klizia.maccaroni87@gmail.com

01/10/2019 – present

01/12/2018–31/09/2019

Post-doctoral training

Supervisor: Professor Isabella Saggio – Dept. of Biology and Biotechnologies “Charles Darwin” 

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy)

Molecular Cytogenetics Laboratory

Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin”

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy) 

01/11/2015–31/10/2018

Ph.D. in Genetics and Molecular Biology (XXXI cycle) – with fellowship  

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy)

Thesis in molecular cytogenetics:

“Common Fragile Sites: updating the causes of their variability in different cell tissues”.

Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin”

Tutor: Doctor Francesca Degrassi – IBPM – CNR – Rome

Coordinator: Professor Fulvio Cruciani - Dept. of Biology and Biotechnologies “Charles Darwin”

EDUCATION AND TRAINING

11/2017–12/2017

Professional Practice Exam in Biology

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy)

10/2013–03/2015

Master’s degree in Genetics and Molecular Biology in Basic and Biomedical Research  

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy)

Master's degree research in molecular cytogenetic (110/110 cum laude):

“Analysis of Common Fragile Sites expression in human lymphocytes and fibroblasts”.

Supervisor: Professor Franca Pelliccia – Dept. of Biology and Biotechnologies “Charles Darwin”

09/2007–07/2012

First degree in Biological Sciences

Sapienza University of Rome

Piazzale Aldo Moro 5, 00185 Rome (Italy)

First degree dissertation in Cellular Biology:

“Telomeres and Telomerase in cellular senescence and cancer”.

Supervisor: Professor Carla Cioni -  Dept. of Biology and Biotechnologies “Charles Darwin”

PERSONAL SKILLS

Native language

Italian

English

Excellent written and oral production. Level B2 certified by First Certificate of English (FCE).

Japanese

Basic written and oral production. Level N5 certified by Japanese Language Proficiency Test (JLPT).

Job-related skills

▪    Cytogenetics: preparation of human karyotypes; recognition and analysis of human normal and pathological chromosomes; analysis of human chromosomal rearrangements and aberrations; Induction and classification of Common Fragile Sites (CFSs) in human cell lines; preparation of cell culture from lymphocytes from peripheral blood; preparation of normal and pathological human cell lines; preparation and analysis of metaphase spreads of human chromosomes; Fluorescence in situ hybridization (FISH) on  human nuclei and metaphases; chromatin fibers preparation through fiber combing technique; R-banding on normal and pathological human karyotypes with Chromomycin A3; Giemsa staining on human chromosomes; G-banding staining on human chromosomes; immunostaining on human fixed chromosomes; preparation of DNA probes with Nick Translation; human nuclei staining with Ag-NOR; observation of metaphases spreads with optic microscopy and fluorescence microscopy with CCD camera; bacterial culture preparation.

▪    Molecular Biology: agarose and polyacrylamide gel electrophoresis; protein extraction and Western Blot; bacterial transformation; DNA extraction (BAC e PAC, plasmids); RNA extraction; real time- PCR; transfection on human cell lines.

▪    Informatics/Bioinformatics: Windows and Macintosh operative systems; Microsoft Office; Adobe Photoshop; Genomic and proteomic databases (NCBI, BLAST, PDB, GO, UniProt, RepeatMasker, OMIM, Mitelman); software and tools for modelling and prediction of proteins’ structure: ExPasy, Pymol 2.0 and PyMod.

ADDITIONAL INFORMATION

Publications

▪    Capitano F., Gargiuli C., Angerilli A., Maccaroni K., Pelliccia F., Mele A., Camilloni G., (2016). RNA polymerase I transcription is modulated by spatial learning in different brain regions. J. Neurochem. 136(4):706-716.

▪    Pelliccia F., Genovesi ML., Maccaroni K., (2015). Fanconi anaemia, chromosome instability, DNA replication and fragile sites. Eur. J Hum Genet, 23, suppl.1, 451.

Poster

▪    Joint meeting AGI-SIMAG – 26-28/09/2019.  Common Fragile Sites: how the impaired replication timing promotes their tissue-specific expression.

Maccaroni K., Balzano E., Mirimao F., Pelliccia F., (2019)

▪    CSHL (NY, USA) – 3-7/09 2019. Eukaryotic DNA Replication & Genome Maintenance.  

Maccaroni K., Balzano E., Mirimao F., Pelliccia F., (2019). Genome Instability at Common Fragile Sites. Updating the causes of their variability in different cell tissues.

Conferences and congresses participation

▪    Cortona (AR) – 26-28/09/2019

Joint Meeting AGI-SIMAG

▪    Cortona (AR) -14 -15/06/2018

Genetics School in Cortona: Cytogenetic and Molecular Citogenomics.

▪    Rome – 18-21/09/2018

XV FISV Congress (Federazione Italiana Scienze della Vita)