Curriculum M. La Torre

Mattia la Torre

 

February 6, 1989, Rogliano (CS), Italy

 

Work address: Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, Italy

Phone: +39 06 49912472            

Fax: +39 06 4456866

E-Mail: mattia.latorre@uniroma1.it

 

 

EDUCATION AND PROFESSIONAL EXPERIENCE:

June 2016 – present: Visiting researcher at Nanyang Technological University (Singapore). Supervisors: Professor Daniela Rhodes, Professor Isabella Saggio. Research work: in tube analysis of AKTIP/Ft1 role at telomeres.

 

November 2015 – present: Post-Doc fellow. Supervisor: Professor Isabella Saggio. Research work: Nuclear architecture and genome metabolism.

 

October 2012 – October 2015: PhD studentship in Life Science. Supervisor: Professor Isabella Saggio. Research work: production and characterization of a mouse line knockout for Ft1, a lamin interacting protein involved in telomere metabolism. In vitro dissection of AKTIP/Ft1 function in the context of telomere metabolism.

 

May 2015 – August 2015: Visiting research fellowship at Nanyang Technological University (Singapore). Supervisors: Professor Isabella Saggio, Professor Daniela Rhodes. Research work: in tube characterization of AKTIP interactions with its binding partners (TRF1, TRF2, PCNA, A-type lamins).

 

October 2012: Laurea cum laude in Applied Biology to Biomedical Research, Sapienza University of Rome. Degree thesis title: “Production of a mouse line knock-out for Ft1 gene, a lamin interactor with effect on telomere metabolism”. Thesis project coordinator: Professor Isabella Saggio, Department of Biology and Biotechnologies "Charles Darwin", La Sapienza.

 

February 2011 – October 2012: Student training in Professor Isabella Saggio laboratory, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome.

 

September 2010: Laurea cum laude in Molecular Biological Science, University of Pisa. Degree thesis title: “Modulation of Cytochromes P450 4A and 2E in organs of control and fed with high fat diet pigs”. Thesis project coordinators: Dr. Vincenzo Longo, Institute of Biology and Agrarian Biotechnology, CNR; Professor Marcella Camici, University of Pisa.

 

2010: Student training, Institute of Biology and Agrarian Biotechnology, CNR, Pisa. Training coordinator Dr. Vincenzo Longo.

 

2009 – 2010: Scholarship for support for person with physical disability, University of Pisa.

 

June 2007: High School Degree (Score 100/100), Amantea Public High School (CS).

 

 

LABORATORY SKILLS

 

·Bacterial Transformation

·Protein expression in bacteria

·Protein purification (size exclusion, ion exchange and affinity chromatography)

·Protein protein interaction assays (GST-pull down, size exclusion chromatography)

·Protein extraction from mammalian cells

·Western Blotting

·2nd generation lentivectors production

·Lentivectors mediated cells transduction

·Mammalian cell culture

·Mammalian cell transfection

·PCR

·Real Time PCR

·RNA extraction from cells and tissues and retro-transcription

·Plasmid DNA Purification (Maxiprep, Midiprep, Miniprep)

·Genomic DNA isolation from cells and tissues

·Immunofluorescence on mammalian cell

·Mouse colony management

·Mouse manipulation

·Genotyping by PCR analysis

·Tissue collection for histology analysis

·Preparation of paraffin and OCT embedded tissues samples for histology analysis

·Preparation of tissue slides for histology analysis

·Hematoxylin and Eosin tissue coloration

 

COMPUTER KNOWLEDGES

 

·
Operating systems: Windows and Mac Os X

·Microsoft Office: Microsoft Word
, Microsoft Excel, 
Microsoft Power Point


·Adobe Suite: Adobe Photoshop CS6, Adobe Bridge CS6

·
Web browsers: Safari, Firefox, Chrome, Internet Explorer

·GeneAmp700

·Quantity one

·GraphPad Prism

·Image J

·Endnote X7

 

 

PARTECIPATION TO SCIENTIFIC CONFERENCES

 

Conference participations

 

Romina Burla, Mattia La Torre, Maria Teresa Carcuro, Grazia Daniela Raffa, Maurizio Gatti, Isabella Saggio. AKTIP (Ft1), a telomeric protein that interacts with lamin, is required for mouse survival and development.

Partecipation at Cold Spring Harbor Laboratory conference, Telomeres and Telomerase, April 28 – May 2, 2015, Cold Spring Harbour, NY, USA

 

Oral presentations

 

Mattia La Torre, Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio. Oral presentation at BeMM symposium 2015, PhD cycle XXVII. “The telomeric protein Ft1 (AKTIP) plays a role in mouse physiology”, 23 January 23, “Sapienza” University of Rome, Rome, Italy

 

Mattia La Torre, Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio. Oral presentation at EMBO Meeting, Telomeres, Telomerase and Disease, “AKTIP, an E2 variant enzyme that interacts with lamin, is required for correct telomere maintenance” , April 30 – May 4, 2014, Brussels, Belgium

 

Posters

Romina Burla, Mattia La Torre, Mariateresa Carcuro, Carla Mottini, Mariarosaria D’Apice, Paola Spitalieri, Giovanna Lattanzi, Maurizio Gatti, Isabella Saggio, “AKTIP (Ft1), a telomeric protein that interacts with lamin, is required for mouse survival and development, Workshop: Current trends in biomedicine, “The nuclear lamina in health and disease”, November 15 – 18, 2015, Baeza, Spain

 

Romina Burla, Mariateresa Carcuro, Mattia la Torre, Grazia D. Raffa, Alessandra Galati, Domenico Raimondo, Angela Rizzo, Laura Ciapponi, Giovanni Cenci, Enrico Cundari, Anna Maria Biroccio, Stefano Cacchione, Maurizio Gatti and Isabella Saggio, “AKTIP, an E2 variant enzyme that interacts with lamin, is required for correct telomere maintenance”, XIII Conference of the Italian Federation of Life Sciences (FISV), September 24 – 27, 2014, Pisa, Italy

 

Romina Burla, Mariateresa Carcuro, Mattia la Torre, Elena Di Matteo, Grazia D. Raffa, Alessandra Galati, Domenico Raimondo, Laura Ciapponi, Giovanni Cenci, Enrico Cundari, Stefano Cacchione, Maurizio Gatti and Isabella Saggio, “AKTIP, an E2 variant enzyme that interacts with lamin and protects mammalian telomeres from replicative damage”, The Progeria Research Foundation scientific workshop, April 24 – 26, 2013, Bethesda, MD, USA

 

 

PUBLICATIONS

 

Romina Burla; Mariateresa Carcuro; Mattia La Torre; Federica Fratini, Marco Crescenzi; Maria Rosaria D’Apice; Paola Spitalieri; GraziaDaniela Raffa; Letizia Astrologo; Giovanna Lattanzi; Enrico Cundari; Domenico Raimondo; Maurizio Gatti; Isabella Saggio, “The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence”, Open Biology, August 2016

 

Romina Burla; Mattia La Torre; Isabella Saggio; “Mammalian telomeres and their partnership with lamins”, Nucleus, April 2016

 

Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Mattia La Torre; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio, “AKTIP/Ft1, a new shelterin-interacting factor required for telomere maintenance’’, Plos Genetics, June 2015

 

Stefania Piersanti, Romina Burla, Valerio Licursi, Catarina Brito, Mattia La Torre, Paula Alves, Daniel Simao, Carla Mottini, Sara Salinas, Rodolfo Negri, Enrico Tagliafico, EJ Kremer, & Isabella Saggio, “Transcriptional response of human neurospheres to helper-dependent CAV-2 vectors: activation of DNA damage response, modulation of microtubule motors and centromeric proteins”, Plos One, July 2015

 

 

 

 

 

NEWS

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