Curriculum M. La Torre

 

Mattia la Torre

 

February 6, 1989, Rogliano (CS), Italy

 

Work address: Department of Biology and Biotechnology “Charles Darwin”,

Sapienza University of Rome, Italy

Phone: +39 06 49912472      

Fax: +39 06 4456866

E-Mail: mattia.latorre@uniroma1.it

 

EDUCATION AND PROFESSIONAL EXPERIENCE:

 

Post-Doc career:

 

April 2019 – present: Post-Doc fellowship at Department of Biology and Biotechnology - Charles Darwin, Sapienza University of Rome, assigned with a scholarship from FIRC – Fondazione Italiana per la Ricerca sul Cancro – AIRC. Supervisor: Professor Isabella Saggio. Research work: Nuclear envelope organization and genome instability in cancer diffusion

 

Jan 2019 – March 2019: Post-Doc fellowship at Department of Biology and Biotechnology - Charles Darwin, Sapienza University of Rome, assigned with a scholarship from Fondazione Adriano Buzzati-Traverso. Supervisor: Professor Isabella Saggio. Research work: Biology and pharmacology of DNA replication stress upstream and downstream pathways in HGPS

 

June 2018 – December 2018: Post-Doc fellowship at Department of Biology and Biotechnology - Charles Darwin, Sapienza University of Rome. Supervisor: Professor Isabella Saggio, funds holder (Progeria Foundation). Research work: AKTIP in progeria

 

The research activity performed contributed to the scientific product:

Romina Burla, Mattia la Torre, Giorgia Zanetti, Alex Bastianelli, Chiara Merigliano, Simona del Giudice, Alessandro Vercelli, Ferdinando Di Cunto, Marina Boido, Fiammetta Vernì and Isabella Saggio. p53-sensitive epileptic behavior and inflammation in Ft1 hypomorphic mice. Frontiers in Genetics, November 2018 ( co-first authorship)

 

Chiara Merigliano, Elisa Mascolo, Mattia la Torre, Isabella Saggio, Fiammetta Vernì. Protective role of vitamin B6 (PLP) against DNA damage in Drosophila models of type 2 diabetes, Scientific reports, July 2018.

 

Romina Burla, Mattia la Torre, Chiara Merigliano, Fiammetta Vernì and Isabella Saggio. Genomic instability and DNA replication defects in progeroid syndromes. Nucleus, June 2018.

 

Mattia la Torre, Chiara Merigliano, Romina Burla, Carla Mottini, Giorgia Zanetti, Simona Del Giudice, Mariateresa Carcuro, Ilaria Virdia, Elisabetta Bucciarelli, Isabella Manni, Gianluca Rampioni Vinciguerra, Giulia Piaggio, Mara Riminucci, Ana Cumano, Armando Bartolazzi, Fiammetta Verni, Silvia Soddu, Maurizio Gatti, Isabella Saggio. Mice with reduced expression of the telomere-associated protein Ft1 develop p53-sensitive progeroid traits. Aging Cell, April 2018 (started during the PhD)

 

May 2017 – April 2018: Post-Doc fellowship at Institute of Molecular Biology and Pathology (IBPM) - National Research Council (CNR) Department of Biology and Biotechnology - Charles Darwin, Sapienza University of Rome. Supervisor: Professor Isabella Saggio; Professor Maurizio Gatti, funds holder (AIRC). Research work: In vivo study of the protein AKTIP/Ft1.

 

May 2016 – April 2017: Post-Doc fellowship at Department of Biology and Biotechnology - Charles Darwin, Sapienza University of Rome. Supervisor: Professor Isabella Saggio, funds holder (Telethon). Research work: In vivo study of the protein AKTIP/Ft1.

 

2015 – present: Active research collaboration with NTU Institute of Structural Biology, Nanynag Technological University (Singapore). Supervisors: Professor Daniela Rhodes and Professor Isabella Saggio. Research work: Analysis of the biochemical properties of the telomeric accessory protein AKTIP/Ft1. Collaboration as research fellow.

 

November 2015 – April 2016: Post-Doc fellow. Supervisor: Professor Isabella Saggio. Research work: Nuclear architecture and genome metabolism.

 

The research activity performed contributed to the scientific products:

Romina Burla; Mariateresa Carcuro; Mattia la Torre; Federica Fratini, Marco Crescenzi; Maria Rosaria D’Apice; Paola Spitalieri; GraziaDaniela Raffa; Letizia Astrologo; Giovanna Lattanzi; Enrico Cundari; Domenico Raimondo; Maurizio Gatti; Isabella Saggio. The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. Open Biology, August 2016

And

Romina Burla; Mattia la Torre; Isabella Saggio. Mammalian telomeres and their partnership with lamins, Nucleus, April 2016

 

PhD career:

October 2012 – October 2015: PhD studentship in Life Science. Supervisor: Professor Isabella Saggio. Research work: production and characterization of a mouse line knockout for Ft1, a lamin interacting protein involved in telomere metabolism. In vitro dissection of AKTIP/Ft1 function in the context of telomere metabolism. Certificate ID 2499125, certified by Dr. Giulietta Capacchione

 

The research activity performed contributed to the scientific products:

Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Mattia la Torre; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio, AKTIP/Ft1, a new shelterin-interacting factor required for telomere maintenance’, Plos Genetics, June 2015

And

Stefania Piersanti, Romina Burla, Valerio Licursi, Catarina Brito, Mattia la Torre, Paula Alves, Daniel Simao, Carla Mottini, Sara Salinas, Rodolfo Negri, Enrico Tagliafico, EJ Kremer, and Isabella Saggio, Transcriptional response of human neurospheres to helper-dependent CAV-2 vectors: activation of DNA damage response, modulation of microtubule motors and centromeric proteins, Plos One, July 2015

 

May 2015 – August 2015: Visiting research fellowship in Nanyang Technological University (Singapore). Supervisors: Professor Isabella Saggio, Professor Daniela Rhodes. Research work: in tube characterization of AKTIP interactions with its binding partners (TRF1, TRF2, PCNA, A-type lamins).

 

Master career: 

October 2012: Laurea cum laude in Applied Biology to Biomedical Research, Sapienza University of Rome. Degree thesis title: “Production of a mouse line knock-out for Ft1 gene, a lamin interactor with effect on telomere metabolism”. Thesis project coordinator: Professor Isabella Saggio, Department of Biology and Biotechnologies "Charles Darwin", La Sapienza. Protocol number 1554431, certified by Dr. Rosalba Natale

 

February 2011 – October 2012: Student training in Professor Isabella Saggio laboratory, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome.

 

Bachelor Degree:

September 2010: Laurea cum laude in Molecular Biological Science, University of Pisa. Degree thesis title: “Modulation of Cytochromes P450 4A and 2E in organs of control and fed with high fat diet pigs”. Thesis project coordinators: Dr. Vincenzo Longo, Institute of Biology and Agrarian Biotechnology, CNR; Professor Marcella Camici, University of Pisa. Protocol number 20111752296, certified by Dr Luigi Rivetti

 

2010: Student training, Institute of Biology and Agrarian Biotechnology, CNR, Pisa. Training coordinator Dr. Vincenzo Longo.

 

2009 – 2010: Scholarship for support for person with physical disability, University of Pisa.

 

 

June 2007: High School Degree (Score 100/100), Amantea Public High School (CS).

 

 

LABORATORY SKILLS

 

-      Bacterial Transformation

-      Protein expression in bacteria

-      Protein purification (size exclusion, ion exchange and affinity chromatography)

-      Protein protein interaction assays (GST-pull down, size exclusion chromatography)

-      Protein DNA interactions (EMSA)

-      Protein extraction from mammalian cells

-      Western Blotting

-      2nd generation lentivectors production

-      Lentivectors mediated cells transduction

-      Mammalian cell culture

-      Mammalian cell transfection

-      PCR

-      Real Time PCR

-      RNA extraction from cells and tissues and retro-transcription

-      Plasmid DNA Purification (Maxiprep, Midiprep, Miniprep)

-      Genomic DNA isolation from cells and tissues

-      Immunofluorescence on mammalian cell

-      Mouse colony management

-      Mouse manipulation

-      Genotyping by PCR analysis

-      Tissue collection for histology analysis

-      Preparation of paraffin and OCT embedded tissues samples for histology analysis

-      Preparation of tissue slides for histology analysis

-      Hematoxylin and Eosin tissue coloration

 

COMPUTER KNOWLEDGES

 

-      Operating systems: Windows and Mac Os X

-      Microsoft Office: Microsoft Word, Microsoft Excel, Microsoft Power Point

-      Adobe Suite: Adobe Photoshop CS6, Adobe Bridge CS6

-      Web browsers: Safari, Firefox, Chrome, Internet Explorer

-      GeneAmp700

-      Quantity one

-      GraphPad Prism

-      Image J

-      Endnote X7

 

PARTECIPATION TO SCIENTIFIC CONFERENCES

 

Conference participations and mentions

 

Romina Burla, Mattia La Torre, Chiara Merigliano, Giorgia Zanetti, Simona Del Giudice, Fiammetta Verni, Daniela Rhodes and Isabella Saggio. Mention in conference, XV FISV Congress Sapienza University of Rome, “A new telomeric and ESCRT complex associated factor”, September 18-21 2018, Rome, Italy

 

Mattia la Torre, Romina Burla, Chiara Merigliano, Teo Hsiang Ling, Liew Chong Wai, Fiammetta Vernì, Daniela Rhodes, Isabella Saggio, “AKTIP a telomeric and ESCRT complex associated factor”. Poster at EMBO Workshop, Telomere biology in health and human disease, May 01-06, 2018, Tróia, Portugal

 

Romina Burla, Mattia la Torre, Giorgia Zanetti, Simona Del Giudice, Chiara Merigliano, Teo Hsiang Ling, Liew Chong Wai, Daniela Rhodes, Maurizio Gatti, Fiammetta Vernì, and Isabella Saggio “Triangle connection of the E2 variant protein AKTIP with lamins and telomeres in generating a p53-sensitive progeroid phenotype”. Poster at Cold Spring Harbor Laboratory, Telomeres and Telomerase, May 02-06, 2017, Cold Spring Harbour, NY, USA

 

Romina Burla, Mattia la Torre, Giorgia Zanetti, Simona Del Giudice, Fiammetta Vernì and Isabella Saggio, “The lamin-interacting protein AKTIP in progeroid syndromes”. 3rd skeleton meeting, March 12-13, 2017 Riva del Garda, Italy

 

Romina Burla, Mattia la Torre, Maria Teresa Carcuro, Grazia Daniela Raffa, Maurizio Gatti, Isabella Saggio. AKTIP (Ft1), a telomeric protein that interacts with lamin, is required for mouse survival and development.

Partecipation at Cold Spring Harbor Laboratory conference, Telomeres and Telomerase, April 28 – May 2, 2015, Cold Spring Harbour, NY, USA

 

Oral presentations

 

Giorgia Zanetti; Romina Burla; Mattia la Torre; Carla Mottini; Simona Del Giudice; Domenico Raimondo; Maurizio Gatti; Isabella Saggio. “AKTIP (Ft1), a protein that interacts with lamins, is required for telomere maintenance and mouse development”. Oral presentation at FISV 2016, September 20-23, “Sapienza” Università di Roma, Roma, Italy

 

Mattia la Torre, Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio. Oral presentation at BeMM symposium 2015, PhD cycle XXVII. “The telomeric protein Ft1 (AKTIP) plays a role in mouse physiology”, 23 January 23, “Sapienza” University of Rome, Rome, Italy

 

Mattia la Torre, Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio. Oral presentation at EMBO Meeting, Telomeres, Telomerase and Disease, “AKTIP, an E2 variant enzyme that interacts with lamin, is required for correct telomere maintenance”, April 30 – May 4, 2014, Brussels, Belgium

 

Posters

 

Romina Burla, Mattia la Torre, Giorgia Zanetti, Simona Del Giudice, Chiara Merigliano, Teo Hsiang Ling, Liew Chong Wai, Daniela Rhodes, Maurizio Gatti, Fiammetta Vernì, and Isabella Saggio “Triangle connection of the E2 variant protein AKTIP with lamins and telomeres in generating a p53-sensitive progeroid phenotype”. Poster presentation Cold Spring Harbor Laboratory, Telomeres and Telomerase, May 02-06, 2017, Cold Spring Harbour, NY, USA

 

Romina Burla, Mattia la Torre, Mariateresa Carcuro, Carla Mottini, Mariarosaria D’Apice, Paola Spitalieri, Giovanna Lattanzi, Maurizio Gatti, Isabella Saggio, “AKTIP (Ft1), a telomeric protein that interacts with lamin, is required for mouse survival and development, Workshop: Current trends in biomedicine, “The nuclear lamina in health and disease”, November 15 – 18, 2015, Baeza, Spain

 

Romina Burla, Mariateresa Carcuro, Mattia la Torre, Grazia D. Raffa, Alessandra Galati, Domenico Raimondo, Angela Rizzo, Laura Ciapponi, Giovanni Cenci, Enrico Cundari, Anna Maria Biroccio, Stefano Cacchione, Maurizio Gatti and Isabella Saggio, “AKTIP, an E2 variant enzyme that interacts with lamin, is required for correct telomere maintenance”, XIII Conference of the Italian Federation of Life Sciences (FISV), September 24 – 27, 2014, Pisa, Italy

 

Romina Burla, Mariateresa Carcuro, Mattia la Torre, Elena Di Matteo, Grazia D. Raffa, Alessandra Galati, Domenico Raimondo, Laura Ciapponi, Giovanni Cenci, Enrico Cundari, Stefano Cacchione, Maurizio Gatti and Isabella Saggio, “AKTIP, an E2 variant enzyme that interacts with lamin and protects mammalian telomeres from replicative damage”, The Progeria Research Foundation scientific workshop, April 24 – 26, 2013, Bethesda, MD, USA

 

PUBLICATIONS

 

Romina Burla, Mattia la Torre, Giorgia Zanetti, Alex Bastianelli, Chiara Merigliano, Simona del Giudice, Alessandro Vercelli, Ferdinando Di Cunto, Marina Boido, Fiammetta Vernì and Isabella Saggio. p53-sensitive epileptic behavior and inflammation in Ft1 hypomorphic mice. Frontiers in Genetics, November 2018 ( co-first authorship)

 

Chiara Merigliano, Elisa Mascolo, Mattia la Torre, Isabella Saggio, Fiammetta Vernì. Protective role of vitamin B6 (PLP) against DNA damage in Drosophila models of type 2 diabetes, Scientific reports, July 2018.

 

Romina Burla, Mattia la Torre, Chiara Merigliano, Fiammetta Vernì and Isabella Saggio. Genomic instability and DNA replication defects in progeroid syndromes. Nucleus, June 2018.

 

Mattia la Torre, Chiara Merigliano, Romina Burla, Carla Mottini, Giorgia Zanetti, Simona Del Giudice, Mariateresa Carcuro, Ilaria Virdia, Elisabetta Bucciarelli, Isabella Manni, Gianluca Rampioni Vinciguerra, Giulia Piaggio, Mara Riminucci, Ana Cumano, Armando Bartolazzi, Fiammetta Verni, Silvia Soddu, Maurizio Gatti, Isabella Saggio. Mice with reduced expression of the telomere-associated protein Ft1 develop p53-sensitive progeroid traits. Aging Cell, April 2018.

 

Romina Burla; Mariateresa Carcuro; Mattia la Torre; Federica Fratini, Marco Crescenzi; Maria Rosaria D’Apice; Paola Spitalieri; GraziaDaniela Raffa; Letizia Astrologo; Giovanna Lattanzi; Enrico Cundari; Domenico Raimondo; Maurizio Gatti; Isabella Saggio. The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. Open Biology, August 2016

 

Romina Burla; Mattia la Torre; Isabella Saggio. Mammalian telomeres and their partnership with lamins, Nucleus, April 2016

 

Romina Burla; Mariateresa Carcuro; Grazia Raffa; Alessandra Galati; Domenico Raimondo; Angela Rizzo; Mattia la Torre; Emanuela Micheli; Laura Ciapponi; Giovanni Cenci; Enrico Cundari; Antonio Musio; Annamaria Biroccio; Stefano Cacchione; Maurizio Gatti; Isabella Saggio, AKTIP/Ft1, a new shelterin-interacting factor required for telomere maintenance’, Plos Genetics, June 2015

 

Stefania Piersanti, Romina Burla, Valerio Licursi, Catarina Brito, Mattia la Torre, Paula Alves, Daniel Simao, Carla Mottini, Sara Salinas, Rodolfo Negri, Enrico Tagliafico, EJ Kremer, and Isabella Saggio, Transcriptional response of human neurospheres to helper-dependent CAV-2 vectors: activation of DNA damage response, modulation of microtubule motors and centromeric proteins, Plos One, July 2015

 

 

FUNDED PROJECTS

2016 - Sapienza Grant PI: Mattia la Torre, number: AR216154C9E53D8D, Analysis of the hematopoietic and stromal BM subsets in mouse models of premature aging

 

Bibliometrics (Scopus June 2019)

h-index: 3

Citations: 41 

SAGGIOLAB NEWS

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Since 2016 an agreement has been signed to promote exchanges between Sapienza and Nanyang Technological University, infos isabella.saggio@uniroma1.it 

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