Curriculum L. Astrologo

Letizia Astrologo
 
January 30, 1987, Rome, Italy
Italian Citizen
 
Work address
Sapienza University of Rome, Department of Biology and Biotechnologies "Charles Darwin" & Department of Molecular Medicine, Rome, Italy
Phone number: +39 0649912849
Fax: +39 064456866
E-mail: letizia.astrologo@uniroma1.it
Skype contact: letizia.astrologo

Education
November 2010-today: PhD studentship in Physiopathology of Skeletal Tissues, Sapienza University of Rome. Supervisor: Prof. Paolo Bianco and Prof. Isabella Saggio

October 2010: Laurea cum laude in Genetics and Molecular Biology, Sapienza University of Rome. Degree Thesis title: "Analysis of transcriptional modulation induced by human and canine adenoviral vectors in human neuronal cells". Supervisor: Prof. Isabella Saggio

December 2008: Laurea cum laude in Biological Sciences, Sapienza University of Rome. Degree Thesis title: "Study of an in vitro model of the genetic disease Fibrous Dysplasia". Supervisor: Prof. Isabella Saggio
 
July 2005: Scientific High School Degree (Score 100/100), Liceo Renzo Levi, Rome, Italy

Professional experiences
2010-today: PhD student, research activity, Stem Cell Laboratory, Sapienza University of Rome. Training coordinators: Prof. Isabella Saggio and Prof. Paolo Bianco. Research work: study of in vitro (mesenchymal stem cells) and in vivo (mouse) models of Fibrous Dysplasia disease through molecular and physiological approaches

2008-2010: Research activity, Gene Therapy Laboratory, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome. Training coordinator: Prof. Isabella Saggio. Research work: high-throughput transcriptional analysis of gene therapy viral vectors effects on brain cells (http://www.braincav.eu)

2008: Student training, Gene Therapy Laboratory, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome. Training coordinator: Prof. Isabella Saggio. Research work: transfer of the Fibrous Dysplasia genotype in human skeletal progenitors through lentiviral vectors and in vitro analysis

2006-2007: Scholarship at the structure of didactic labs of Department of Animal and Human Biology (BAU), Sapienza University of Rome

Laboratory skills
Cell cultures (primary, stem cells and cell lines)
Cell transfection
Lentiviral vector production and titration
Lentivirus-mediated transduction
Adenoviral vector production and titration
Adenovirus-mediated transduction
Genomic DNA isolation from cells and tissues
RNA extraction from cells and tissues and retrotranscription
PCR, RT-PCR, Real Time PCR
Protein extraction from cells
Western Blotting
Bacterial transformation
Plasmid DNA purification (Maxiprep, Midiprep)
ELISA assay
Mouse management and manipulation

Computer Knowledge (Windows, Mac)
Browser web: Microsoft Internet Explorer, Firefox, Safari, Google Chrome
Microsoft Office package
GraphPad Prism
Adobe Photoshop CS5.1
DNAman
ApE – A plasmid Editor
Quantity one
MetaMorph
Endnote 9
GenoPro
Microarray analysis: Expression Analysis Sistematic Explorer, Ingenuity Pathway Analysis

Foreign Languages
English, Hebrew

Posters and presentations
Piersanti S., Astrologo L., Schwarz S.C., Schwarz J., Chillon M., Kremer E.J., Saggio I. High-throughput transcriptional analysis of gene therapy viral vectors effects on brain cells. Poster, 18th European Society of Gene and Cell Therapy Annual Congress 2010. Milan, Italy (October 2010)
 
Piersanti S., Astrologo L., Licursi V., Negri R., Roncaglia E., TagliaficoE., Schwarz S., Schwarz J., Kremer E.J., Saggio I. Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in human dopaminergic neurons. Poster, 10thInternational Adenovirus Meeting. Umeå, Sweden (June 2012)
 
Astrologo L., Piersanti S., Remoli C., Costa R., Tagliafico E., Roncaglia E., Funari A., SacchettiB., Riminucci M., Saggio I., Bianco P. High-throughput analysis of downstream effects of activating Gsa mutations in skeletal progenitor/stem cells. Oral communication, FISV 2012, 12th Congress. Rome, Italy (September 2012)
 
Piersanti S., Astrologo L., Licursi V., Negri R., Roncaglia E., Tagliafico E., Schwarz S., SchwarzJ., Kremer E.J., Saggio I. Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in dopaminergic differentiated human midbrain neuronal progenitors. Poster, FISV 2012, 12th Congress. Rome, Italy (September 2012)
 
Piersanti S., Astrologo L., Licursi V., Negri R., Roncaglia E., Tagliafico E., Schwarz S., Schwarz J., Kremer E.J., Saggio I. Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in dopaminergic differentiated human midbrain neuronal progenitors. Poster, 20th European Society of Gene and Cell Therapy Annual Congress 2012. Versailles, France (October 2012)