Curriculum C. Remoli

Personal Details

Cristina Remoli

Born: Rome (Italy), March 9, 1978.

Nationality: Italian

Marital status: Unmarried

Languages: Italian, English

Professional address: Institute of Cell Biology and Tissue Engineering

                               San Raffaele Biomedical Science Park of Rome

                                   Via di Castel Romano 100

                                   00128, Rome, Italy

(www.scienceparkrome.org)

Phone: +39 06 80319029

Fax: +39 06 80319054

E-mail: [email protected]

2006-today: PhD student in Physiopathology and Clinic of Skeletric Apparatus, University of Rome “La Sapienza”.

2005: Laurea cum laude in Biological Sciences, Biotechnology specialization. University of Rome “La Sapienza”. Degree thesis title: “Expression and interference of GNASR201C mutant in skeletal stem cells. A model for studying and therapy of Fibrous Dysplasia”.

2000-2005: Graduate studies at the University of Rome, La Sapienza, Biological Sciences.

1996-2000: Graduate studies at the University of Rome, La Sapienza, Medicine and Surgery.

1996: Maturità Scientifica (High School Degree) Liceo Scientifico A. Labriola, Rome, Italy

2006-today: PhD student in Physiopathology and Clinic of Skeletric Apparatus, University of Rome “La Sapienza”/ Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical Science Park of Rome

Supervisor: Prof. Paolo Bianco

PhD coordinator: Prof. Roberto Scandurra

2004-2006: Student training, Department of Genetics and Molecular Biology, University of Rome, “La Sapienza”. Training coordinator Prof. Isabella Saggio.

1. S. Piersanti, I. Saggio, A. Funari, C. Remoli, S. Cersosimo, P.G. Robey, M. Riminucci, P. Bianco. Modeling and correcting fibrous dysplasia with lentiviral vectors. Poster FISV 2008, X Annual Congress. Riva del Garda, Italy (September 2008).

1. Caruso P., Burla R., Martina Y., Piersanti S., Remoli C., Saggio I. Adenovirus-induced activation of the human prion. Poster, FISV 2007 IX annual congress. Riva del Garda, Italy. (September 2007)

1. Burla R., Caruso P., Piersanti S., Remoli C., Cundari E., Saggio I. Knock down of ATM and ATR induces p53 displacement from centrosomes in human primary cells. Poster, FISV 2007 IX annual congress. Riva del Garda, Italy. (September 2007)

1. S. Michienzi, A. Funari, B. Sacchetti, S. Cersosimo, S. Piersanti, C. Remoli, I. Saggio, M. Riminucci and P. Bianco. FGF-2 and TGFβ-1 have opposite effects on the mural cell phenotype of CD146+ BMSCs. Poster, Genostem, 3nd Workshop on Mesenchymal Stem Cells Engineering for Connective Tissue Disorders. Barcelona, Spain. (February 2007)

1. B. Sacchetti, A. Funari, S. Michienzi, G. Giannicola, S. Cersosimo, S. Piersanti, C. Remoli, I. Saggio, P. Robey, M. Riminucci and P. Bianco. Prospective isolation of MCAM/CD146-expressing, clonogenic cells endowed with high spontaneous myogenic activity from the microvasculature of human adult skeletal muscle. Poster, Genostem, 3nd Workshop on Mesenchymal Stem Cells Engineering for Connective Tissue Disorders. Barcelona, Spain. (February 2007)

1. Funari, S. Michienzi, B. Sacchetti, S. Cersosimo, S. Piersanti, C. Remoli, I. saggio, M. Riminucci and P. Bianco. The potential role of CD146+ stromal cells in the assembly and remodeling of pseudovascular structures and in the establishment of HSC niche. Poster, Genostem, 3nd Workshop on Mesenchymal Stem Cells Engineering for Connective Tissue Disorders. Barcelona, Spain. (February 2007)

1. S. Piersanti, B. Sacchetti, A. Funari, C. Remoli, S. Michienzi, M. Riminucci, I. Saggio, P. Bianco. Transfer and silencing of a disease gene (R201C-gnas) in human skeletal stem cells (sscs). Poster, XXXIII European Symposium on Calcified Tissues, Praga. (May 2006)

Pubblications

1.   Piersanti S, Remoli C, Saggio I, Funari A, Michienzi S, Sacchetti B, Gehron Robey P, Riminucci M and Bianco P. Transfer, analysis and reversion of the fibrous dysplasia cellular phenotype in human skeletal progenitors. J Bone Miner Res. In press.

2.   Caruso P, Burla R, Piersanti S, Cherubini G, Remoli C, Martina Y, Saggio I. Prion expression is activated by Adenovirus 5 infection and affects the adenoviral cycle in human cells.Virology. 2009 Mar 15;385(2):343-50. 

Technical skills

Bench skills:

• Genomic DNA isolation from cells and tissues
• RNA extraction from cells and tissues and retrotranscription
• RT-PCR
• Cloning techniques
• Plasmid DNA Purification (Maxiprep, Midiprep, Miniprep)
• Bacterial Transformation
• Protein extraction from cells and tissues.
• Western Blotting
• ELISA
• Southern Blotting
• Cell culture
• Cell Transfection
• Lentiviral vector production
• Lentivirus-mediated transduction
• Real Time PCR
• Microbiology techniques
• Mouse manipulation
  
  

Computer skills:

·         Microsoft Office applications

·         Internet Explorer

·         QuantityOne software (1D- electrophoresis gels analysis)

·         Primer Express (Applications-based Primer Design Software)

·         Adobe Photoshop applications

·         DNAMAN (sequence analysis)

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